Development of a fluorescence resonance energy transfer assay for monitoring bacterial collagenase triple-helical peptidase activity

Due to their efficiency in the hydrolysis of the collagen triple-helix, Clostridial histolyticum collagenases are utilized for isolation of cells from various tissues, including isolation of the human pancreatic islets. However, the instability of Clostridial collagenase I (Col G) results in a degra...

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Bibliographic Details
Published in:Anal Biochem
Main Authors: Tokmina-Roszyk, Michal, Tokmina-Roszyk, Dorota, Bhowmick, Manishabrata, Fields, Gregg B.
Format: Artigo
Language:Inglês
Published: 2014
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4260936/
https://ncbi.nlm.nih.gov/pubmed/24608089
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2014.02.024
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