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Development of a fluorescence resonance energy transfer assay for monitoring bacterial collagenase triple-helical peptidase activity
Due to their efficiency in the hydrolysis of the collagen triple-helix, Clostridial histolyticum collagenases are utilized for isolation of cells from various tissues, including isolation of the human pancreatic islets. However, the instability of Clostridial collagenase I (Col G) results in a degra...
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| Veröffentlicht in: | Anal Biochem |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4260936/ https://ncbi.nlm.nih.gov/pubmed/24608089 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2014.02.024 |
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