Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter

Genomic engineering by the guide RNA (gRNA)-directed CRISPR/CAS9 is rapidly becoming a method of choice for various biological systems. However, pressing concerns remain regarding its off-target activities and wide variations in efficacies. While next generation sequencing (NGS) has been primarily u...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Biotechnol
Main Authors: Zhang, Jian-Hua, Pandey, Mritunjay, Kahler, John F., Loshakov, Anna, Harris, Benjamin, Dagur, Pradeep K., Mo, Yinyuan, Simonds, William F.
Format: Artigo
Jezik:Inglês
Izdano: 2014
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4252756/
https://ncbi.nlm.nih.gov/pubmed/25193712
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jbiotec.2014.08.033
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