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Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter
Genomic engineering by the guide RNA (gRNA)-directed CRISPR/CAS9 is rapidly becoming a method of choice for various biological systems. However, pressing concerns remain regarding its off-target activities and wide variations in efficacies. While next generation sequencing (NGS) has been primarily u...
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| Publicado no: | J Biotechnol |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4252756/ https://ncbi.nlm.nih.gov/pubmed/25193712 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jbiotec.2014.08.033 |
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