In vitro, long-range sequence information for de novo genome assembly via transposase contiguity

We describe a method that exploits contiguity preserving transposase sequencing (CPT-seq) to facilitate the scaffolding of de novo genome assemblies. CPT-seq is an entirely in vitro means of generating libraries comprised of 9216 indexed pools, each of which contains thousands of sparsely sequenced...

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Detalles Bibliográficos
Publicado en:Genome Res
Main Authors: Adey, Andrew, Kitzman, Jacob O., Burton, Joshua N., Daza, Riza, Kumar, Akash, Christiansen, Lena, Ronaghi, Mostafa, Amini, Sasan, L. Gunderson, Kevin, Steemers, Frank J., Shendure, Jay
Formato: Artigo
Idioma:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2014
Assuntos:
Method
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4248320/
https://ncbi.nlm.nih.gov/pubmed/25327137
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.178319.114
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