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Fluorescence lifetime plate reader: Resolution and precision meet high-throughput

We describe a nanosecond time-resolved fluorescence spectrometer that acquires fluorescence decay waveforms from each well of a 384-well microplate in 3 min with signal-to-noise exceeding 400 using direct waveform recording. The instrument combines high-energy pulsed laser sources (5–10 kHz repetiti...

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Bibliografiske detaljer
Udgivet i:Rev Sci Instrum
Main Authors: Petersen, Karl J., Peterson, Kurt C., Muretta, Joseph M., Higgins, Sutton E., Gillispie, Gregory D., Thomas, David D.
Format: Artigo
Sprog:Inglês
Udgivet: American Institute of Physics 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4242087/
https://ncbi.nlm.nih.gov/pubmed/25430092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4900727
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