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A fluorescent plate reader assay for ceramide kinase

Ceramide kinase and its product ceramide 1-phosphate have been implicated in cellular proliferation and survival, activation of cytosolic phospholipase A(2), mast cell degranulation, and phagocytosis. Current assays for ceramide kinase activity employ [(32)P]-ATP, with separation of labelled product...

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Detalhes bibliográficos
Main Authors: Don, Anthony S, Rosen, Hugh
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2516974/
https://ncbi.nlm.nih.gov/pubmed/18206978
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.12.024
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