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A Simple and Sensitive Assay for Ascorbate Using a Plate Reader

We have developed a rapid, inexpensive, and reliable assay for the determination of ascorbate using a plate reader. In this assay, ascorbic acid is oxidized to dehydroascorbic using Tempol and then reacted with o-phenylenediamine to form the condensation product, 3-(dihydroxyethyl)furo[3,4-b]quinoxa...

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Detalhes bibliográficos
Main Authors: Vislisel, Jesse M., Schafer, Freya Q., Buettner, Garry R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2129083/
https://ncbi.nlm.nih.gov/pubmed/17433246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.03.002
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