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Accurate comparison of antibody expression levels by reproducible transgene targeting in engineered recombination-competent CHO cells
Over the years, Chinese hamster ovary (CHO) cells have emerged as the major host for expressing biotherapeutic proteins. Traditional methods to generate high-producer cell lines rely on random integration(s) of the gene of interest but have thereby left the identification of bottlenecks as a challen...
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| 出版年: | Appl Microbiol Biotechnol |
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| 主要な著者: | , , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Springer Berlin Heidelberg
2014
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4231286/ https://ncbi.nlm.nih.gov/pubmed/25158835 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-014-6011-1 |
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