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Transgene copy number comparison in recombinant mammalian cell lines: critical reflection of quantitative real-time PCR evaluation
Nucleic acid quantification is a relevant issue for the characterization of mammalian recombinant cell lines and also for the registration of producer clones. Quantitative real-time PCR is a powerful tool to investigate nucleic acid levels but numerous different quantification strategies exist, whic...
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| Main Authors: | , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Springer Netherlands
2013
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3967612/ https://ncbi.nlm.nih.gov/pubmed/23807595 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-013-9606-y |
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