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Transgene copy number comparison in recombinant mammalian cell lines: critical reflection of quantitative real-time PCR evaluation

Nucleic acid quantification is a relevant issue for the characterization of mammalian recombinant cell lines and also for the registration of producer clones. Quantitative real-time PCR is a powerful tool to investigate nucleic acid levels but numerous different quantification strategies exist, whic...

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Dettagli Bibliografici
Autori principali: Sommeregger, Wolfgang, Prewein, Bernhard, Reinhart, David, Mader, Alexander, Kunert, Renate
Natura: Artigo
Lingua:Inglês
Pubblicazione: Springer Netherlands 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3967612/
https://ncbi.nlm.nih.gov/pubmed/23807595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-013-9606-y
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