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DNA sequencing by synthesis using 3′-O-azidomethyl nucleotide reversible terminators and surface-enhanced Raman spectroscopic detection
As an alternative to fluorescence-based DNA sequencing by synthesis (SBS), we report here an approach using an azido moiety (N(3)) that has an intense, narrow and unique Raman shift at 2125 cm(−1), where virtually all biological molecules are transparent, as a label for SBS. We first demonstrated th...
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| Publicado en: | RSC Adv |
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| Autores principales: | , , , , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2014
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4226440/ https://ncbi.nlm.nih.gov/pubmed/25396047 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/C4RA08398A |
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