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Generation of a Single Chain Antibody Variable Fragment (scFv) to Sense Selectively RhoB Activation

Determining the cellular level of activated form of RhoGTPases is of key importance to understand their regulatory functions in cell physiopathology. We previously reported scFvC1, that selectively bind to the GTP-bound form of RhoA, RhoB and RhoC. In this present study we generate, by molecular evo...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Chinestra, Patrick, Olichon, Aurélien, Medale-Giamarchi, Claire, Lajoie-Mazenc, Isabelle, Gence, Rémi, Inard, Cyril, Ligat, Laetitia, Faye, Jean-Charles, Favre, Gilles
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4218836/
https://ncbi.nlm.nih.gov/pubmed/25365345
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0111034
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