DNA Mapping Using Microfluidic Stretching and Single-Molecule Detection of Fluorescent Site-Specific Tags

We have developed a rapid molecular mapping technology—Direct Linear Analysis (DLA)—on the basis of the analysis of individual DNA molecules bound with sequence-specific fluorescent tags. The apparatus includes a microfluidic device for stretching DNA molecules in elongational flow that is coupled t...

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Detalles Bibliográficos
Publicado en:Genome Res
Autores principales: Chan, Eugene Y., Goncalves, Nuno M., Haeusler, Rebecca A., Hatch, Amie J., Larson, Jonathan W., Maletta, Anthony M., Yantz, Gregory R., Carstea, Eugene D., Fuchs, Martin, Wong, Gordon G., Gullans, Steven R., Gilmanshin, Rudolf
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2004
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC419792/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15173119/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.1635204
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