DNA Mapping Using Microfluidic Stretching and Single-Molecule Detection of Fluorescent Site-Specific Tags

We have developed a rapid molecular mapping technology—Direct Linear Analysis (DLA)—on the basis of the analysis of individual DNA molecules bound with sequence-specific fluorescent tags. The apparatus includes a microfluidic device for stretching DNA molecules in elongational flow that is coupled t...

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Detalhes bibliográficos
Main Authors: Chan, Eugene Y., Goncalves, Nuno M., Haeusler, Rebecca A., Hatch, Amie J., Larson, Jonathan W., Maletta, Anthony M., Yantz, Gregory R., Carstea, Eugene D., Fuchs, Martin, Wong, Gordon G., Gullans, Steven R., Gilmanshin, Rudolf
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2004
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC419792/
https://ncbi.nlm.nih.gov/pubmed/15173119
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.1635204
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