Selective Microbial Genomic DNA Isolation Using Restriction Endonucleases

To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI G(m6)...

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Detalles Bibliográficos
Main Authors: Barnes, Helen E., Liu, Guohong, Weston, Christopher Q., King, Paula, Pham, Long K., Waltz, Shannon, Helzer, Kimberly T., Day, Laura, Sphar, Dan, Yamamoto, Robert T., Forsyth, R. Allyn
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2014
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4184833/
https://ncbi.nlm.nih.gov/pubmed/25279840
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0109061
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