Selective Microbial Genomic DNA Isolation Using Restriction Endonucleases

To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI G(m6)...

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Autori principali: Barnes, Helen E., Liu, Guohong, Weston, Christopher Q., King, Paula, Pham, Long K., Waltz, Shannon, Helzer, Kimberly T., Day, Laura, Sphar, Dan, Yamamoto, Robert T., Forsyth, R. Allyn
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2014
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4184833/
https://ncbi.nlm.nih.gov/pubmed/25279840
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0109061
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