Selective Microbial Genomic DNA Isolation Using Restriction Endonucleases

To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI G(m6)...

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Detalhes bibliográficos
Main Authors: Barnes, Helen E., Liu, Guohong, Weston, Christopher Q., King, Paula, Pham, Long K., Waltz, Shannon, Helzer, Kimberly T., Day, Laura, Sphar, Dan, Yamamoto, Robert T., Forsyth, R. Allyn
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4184833/
https://ncbi.nlm.nih.gov/pubmed/25279840
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0109061
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