Epigenetic Segregation of Microbial Genomes from Complex Samples Using Restriction Endonucleases HpaII and McrB

We describe continuing work to develop restriction endonucleases as tools to enrich targeted genomes of interest from diverse populations. Two approaches were developed in parallel to segregate genomic DNA based on cytosine methylation. First, the methyl-sensitive endonuclease HpaII was used to bind...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Liu, Guohong, Weston, Christopher Q., Pham, Long K., Waltz, Shannon, Barnes, Helen, King, Paula, Sphar, Dan, Yamamoto, Robert T., Forsyth, R. Allyn
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4699840/
https://ncbi.nlm.nih.gov/pubmed/26727463
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0146064
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