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Epigenetic Segregation of Microbial Genomes from Complex Samples Using Restriction Endonucleases HpaII and McrB.

We describe continuing work to develop restriction endonucleases as tools to enrich targeted genomes of interest from diverse populations. Two approaches were developed in parallel to segregate genomic DNA based on cytosine methylation. First, the methyl-sensitive endonuclease HpaII was used to bind...

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Detalhes bibliográficos
Main Authors: Guohong Liu, Christopher Q Weston, Long K Pham, Shannon Waltz, Helen Barnes, Paula King, Dan Sphar, Robert T Yamamoto, R Allyn Forsyth
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science (PLoS) 2016-01-01
Colecção:PLoS ONE
Acesso em linha:http://europepmc.org/articles/PMC4699840?pdf=render
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