Selective Microbial Genomic DNA Isolation Using Restriction Endonucleases

To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI G(m6)...

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Main Authors: Barnes, Helen E., Liu, Guohong, Weston, Christopher Q., King, Paula, Pham, Long K., Waltz, Shannon, Helzer, Kimberly T., Day, Laura, Sphar, Dan, Yamamoto, Robert T., Forsyth, R. Allyn
格式: Artigo
語言:Inglês
出版: Public Library of Science 2014
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Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4184833/
https://ncbi.nlm.nih.gov/pubmed/25279840
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0109061
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