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Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH

Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes...

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Bibliografiske detaljer
Main Authors: Basu, Reelina, Lai, Lan-Tian, Meng, Zhenyu, Wu, Jun, Shao, Fangwei, Zhang, Li-Feng
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4167323/
https://ncbi.nlm.nih.gov/pubmed/25226542
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0107425
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