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PEG-Labeled Nucleotides and Nanopore Detection for Single Molecule DNA Sequencing by Synthesis
We describe a novel single molecule nanopore-based sequencing by synthesis (Nano-SBS) strategy that can accurately distinguish four bases by detecting 4 different sized tags released from 5′-phosphate-modified nucleotides. The basic principle is as follows. As each nucleotide is incorporated into th...
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| Main Authors: | , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3448304/ https://ncbi.nlm.nih.gov/pubmed/23002425 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep00684 |
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