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PEG-Labeled Nucleotides and Nanopore Detection for Single Molecule DNA Sequencing by Synthesis

We describe a novel single molecule nanopore-based sequencing by synthesis (Nano-SBS) strategy that can accurately distinguish four bases by detecting 4 different sized tags released from 5′-phosphate-modified nucleotides. The basic principle is as follows. As each nucleotide is incorporated into th...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Kumar, Shiv, Tao, Chuanjuan, Chien, Minchen, Hellner, Brittney, Balijepalli, Arvind, Robertson, Joseph W. F., Li, Zengmin, Russo, James J., Reiner, Joseph E., Kasianowicz, John J., Ju, Jingyue
التنسيق: Artigo
اللغة:Inglês
منشور في: Nature Publishing Group 2012
الموضوعات:
الوصول للمادة أونلاين:https://ncbi.nlm.nih.gov/pmc/articles/PMC3448304/
https://ncbi.nlm.nih.gov/pubmed/23002425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep00684
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