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Characterization of a Ras mutant with identical GDP- and GTP-bound structures

We previously characterized the G60A mutant of Ras and showed that the switch regions of the GTP- but not the GDP-bound form of this mutant adopt an ‘open conformation’ similar to that seen in nucleotide free Ras. Here, we mutate Lys147 of the conserved (145)SAK(147) motif in the G60A background and...

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Detalhes bibliográficos
Main Authors: Ford, Bradley, Boykevisch, Sean, Zhao, Chen, Kunzelmann, Simone, Bar-Sagi, Dafna, Herrmann, Christian, Nassar, Nicolas
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4160238/
https://ncbi.nlm.nih.gov/pubmed/19883123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi901479b
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