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Characterization of a Ras mutant with identical GDP- and GTP-bound structures

We previously characterized the G60A mutant of Ras and showed that the switch regions of the GTP- but not the GDP-bound form of this mutant adopt an ‘open conformation’ similar to that seen in nucleotide free Ras. Here, we mutate Lys147 of the conserved (145)SAK(147) motif in the G60A background and...

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Autors principals: Ford, Bradley, Boykevisch, Sean, Zhao, Chen, Kunzelmann, Simone, Bar-Sagi, Dafna, Herrmann, Christian, Nassar, Nicolas
Format: Artigo
Idioma:Inglês
Publicat: 2009
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4160238/
https://ncbi.nlm.nih.gov/pubmed/19883123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi901479b
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