Single-step Enrichment by Ti4(+)-IMAC and Label-free Quantitation Enables In-depth Monitoring of Phosphorylation Dynamics with High Reproducibility and Temporal Resolution

Quantitative phosphoproteomics workflows traditionally involve additional sample labeling and fractionation steps for accurate and in-depth analysis. Here we report a high-throughput, straightforward, and comprehensive label-free phosphoproteomics approach using the highly selective, reproducible, a...

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Bibliographische Detailangaben
Hauptverfasser: de Graaf, Erik L., Giansanti, Piero, Altelaar, A. F. Maarten, Heck, Albert J. R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Biochemistry and Molecular Biology 2014
Schlagworte:
Technological Innovation and Resources
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4159659/
https://ncbi.nlm.nih.gov/pubmed/24850871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O113.036608
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