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Defeating Major Contaminants in Fe(3+)- Immobilized Metal Ion Affinity Chromatography (IMAC) Phosphopeptide Enrichment

Here we demonstrate that biomolecular contaminants, such as nucleic acid molecules, can seriously interfere with immobilized metal ion affinity chromatography (IMAC)-based phosphopeptide enrichments. We address and largely solve this issue, developing a robust protocol implementing methanol/chlorofo...

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Detalhes bibliográficos
Publicado no:Mol Cell Proteomics
Main Authors: Potel, Clement M., Lin, Miao-Hsia, Heck, Albert J. R., Lemeer, Simone
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5930410/
https://ncbi.nlm.nih.gov/pubmed/29449344
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR117.000518
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