Defeating Major Contaminants in Fe(3+)- Immobilized Metal Ion Affinity Chromatography (IMAC) Phosphopeptide Enrichment

Here we demonstrate that biomolecular contaminants, such as nucleic acid molecules, can seriously interfere with immobilized metal ion affinity chromatography (IMAC)-based phosphopeptide enrichments. We address and largely solve this issue, developing a robust protocol implementing methanol/chlorofo...

Full description

Saved in:
Bibliographic Details
Published in:Mol Cell Proteomics
Main Authors: Potel, Clement M., Lin, Miao-Hsia, Heck, Albert J. R., Lemeer, Simone
Format: Artigo
Language:Inglês
Published: The American Society for Biochemistry and Molecular Biology 2018
Subjects:
Technological Innovation and Resources
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5930410/
https://ncbi.nlm.nih.gov/pubmed/29449344
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR117.000518
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items