Загрузка...
Defeating Major Contaminants in Fe(3+)- Immobilized Metal Ion Affinity Chromatography (IMAC) Phosphopeptide Enrichment
Here we demonstrate that biomolecular contaminants, such as nucleic acid molecules, can seriously interfere with immobilized metal ion affinity chromatography (IMAC)-based phosphopeptide enrichments. We address and largely solve this issue, developing a robust protocol implementing methanol/chlorofo...
Сохранить в:
| Опубликовано в: : | Mol Cell Proteomics |
|---|---|
| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
The American Society for Biochemistry and Molecular Biology
2018
|
| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5930410/ https://ncbi.nlm.nih.gov/pubmed/29449344 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR117.000518 |
| Метки: |
Добавить метку
Нет меток, Требуется 1-ая метка записи!
|