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Comparing Multi-Step IMAC and Multi-Step TiO(2) Methods for Phosphopeptide Enrichment

Phosphopeptide enrichment from complicated peptide mixtures is an essential step for mass spectrometry-based phosphoproteomic studies to reduce sample complexity and ionization suppression effects. Typical methods for enriching phosphopeptides include immobilized metal affinity chromatography (IMAC)...

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Detalhes bibliográficos
Publicado no:Anal Chem
Main Authors: Yue, Xiaoshan, Schunter, Alissa, Hummon, Amanda B.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4766865/
https://ncbi.nlm.nih.gov/pubmed/26237447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.5b01833
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