Single-step Enrichment by Ti4(+)-IMAC and Label-free Quantitation Enables In-depth Monitoring of Phosphorylation Dynamics with High Reproducibility and Temporal Resolution

Quantitative phosphoproteomics workflows traditionally involve additional sample labeling and fractionation steps for accurate and in-depth analysis. Here we report a high-throughput, straightforward, and comprehensive label-free phosphoproteomics approach using the highly selective, reproducible, a...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: de Graaf, Erik L., Giansanti, Piero, Altelaar, A. F. Maarten, Heck, Albert J. R.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: The American Society for Biochemistry and Molecular Biology 2014
Gaiak:
Technological Innovation and Resources
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4159659/
https://ncbi.nlm.nih.gov/pubmed/24850871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O113.036608
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