Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning

[Image: see text] Using a set of six (1)H-detected triple-resonance NMR experiments, we establish a method for sequence-specific backbone resonance assignment of magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectra of 5–30 kDa proteins. The approach relies on perdeuteration, amide (2)...

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Autors principals: Barbet-Massin, Emeline, Pell, Andrew J., Retel, Joren S., Andreas, Loren B., Jaudzems, Kristaps, Franks, W. Trent, Nieuwkoop, Andrew J., Hiller, Matthias, Higman, Victoria, Guerry, Paul, Bertarello, Andrea, Knight, Michael J., Felletti, Michele, Le Marchand, Tanguy, Kotelovica, Svetlana, Akopjana, Inara, Tars, Kaspars, Stoppini, Monica, Bellotti, Vittorio, Bolognesi, Martino, Ricagno, Stefano, Chou, James J., Griffin, Robert G., Oschkinat, Hartmut, Lesage, Anne, Emsley, Lyndon, Herrmann, Torsten, Pintacuda, Guido
Format: Artigo
Idioma:Inglês
Publicat: American Chemical Society 2014
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4156866/
https://ncbi.nlm.nih.gov/pubmed/25102442
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja507382j
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