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Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning
[Image: see text] Using a set of six (1)H-detected triple-resonance NMR experiments, we establish a method for sequence-specific backbone resonance assignment of magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectra of 5–30 kDa proteins. The approach relies on perdeuteration, amide (2)...
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
American Chemical
Society
2014
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4156866/ https://ncbi.nlm.nih.gov/pubmed/25102442 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja507382j |
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