Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning

[Image: see text] Using a set of six (1)H-detected triple-resonance NMR experiments, we establish a method for sequence-specific backbone resonance assignment of magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectra of 5–30 kDa proteins. The approach relies on perdeuteration, amide (2)...

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Päätekijät: Barbet-Massin, Emeline, Pell, Andrew J., Retel, Joren S., Andreas, Loren B., Jaudzems, Kristaps, Franks, W. Trent, Nieuwkoop, Andrew J., Hiller, Matthias, Higman, Victoria, Guerry, Paul, Bertarello, Andrea, Knight, Michael J., Felletti, Michele, Le Marchand, Tanguy, Kotelovica, Svetlana, Akopjana, Inara, Tars, Kaspars, Stoppini, Monica, Bellotti, Vittorio, Bolognesi, Martino, Ricagno, Stefano, Chou, James J., Griffin, Robert G., Oschkinat, Hartmut, Lesage, Anne, Emsley, Lyndon, Herrmann, Torsten, Pintacuda, Guido
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Chemical Society 2014
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4156866/
https://ncbi.nlm.nih.gov/pubmed/25102442
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja507382j
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