Rational Design, Preparation, and Characterization of a Therapeutic Enzyme Mutant with Improved Stability and Function for Cocaine Detoxification

[Image: see text] Cocaine esterase (CocE) is known as the most efficient natural enzyme for cocaine hydrolysis. The major obstacle to the clinical application of wild-type CocE is the thermoinstability with a half-life of only ∼12 min at 37 °C. The previously designed T172R/G173Q mutant (denoted as...

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Hauptverfasser: Fang, Lei, Chow, K. Martin, Hou, Shurong, Xue, Liu, Chen, Xiabin, Rodgers, David W., Zheng, Fang, Zhan, Chang-Guo
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Chemical Society 2014
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4136690/
https://ncbi.nlm.nih.gov/pubmed/24919140
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cb500257s
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