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Mutational analysis of a type II topoisomerase cleavage site: distinct requirements for enzyme and inhibitors.
We have analyzed the DNA sequence requirements for cleavage of a 30 bp oligonucleotide that contains a strong bacteriophage T4 type II topoisomerase site. A novel method was used to generate substrates with each of the four nucleotides at 10 positions surrounding the cleavage site, and mutant substr...
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| Publicado no: | EMBO J |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC413430/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8387918/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1993.tb05857.x |
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