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Mutational analysis of a type II topoisomerase cleavage site: distinct requirements for enzyme and inhibitors.

We have analyzed the DNA sequence requirements for cleavage of a 30 bp oligonucleotide that contains a strong bacteriophage T4 type II topoisomerase site. A novel method was used to generate substrates with each of the four nucleotides at 10 positions surrounding the cleavage site, and mutant substr...

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Autors principals: Freudenreich, C H, Kreuzer, K N
Format: Artigo
Idioma:Inglês
Publicat: 1993
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC413430/
https://ncbi.nlm.nih.gov/pubmed/8387918
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