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Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines
Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation ‘Tcarbs’) after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glyco...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4133772/ https://ncbi.nlm.nih.gov/pubmed/23196974 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.138 |
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