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In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry
Here we describe the application of a new click chemistry method for fluorescent tracking of protein synthesis in individual microorganisms within environmental samples. This technique, termed bioorthogonal non-canonical amino acid tagging (BONCAT), is based on the in vivo incorporation of the non-c...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BlackWell Publishing Ltd
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4122687/ https://ncbi.nlm.nih.gov/pubmed/24571640 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1462-2920.12436 |
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