In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry

Here we describe the application of a new click chemistry method for fluorescent tracking of protein synthesis in individual microorganisms within environmental samples. This technique, termed bioorthogonal non-canonical amino acid tagging (BONCAT), is based on the in vivo incorporation of the non-c...

Description complète

Enregistré dans:
Détails bibliographiques
Auteurs principaux: Hatzenpichler, Roland, Scheller, Silvan, Tavormina, Patricia L, Babin, Brett M, Tirrell, David A, Orphan, Victoria J
Format: Artigo
Langue:Inglês
Publié: BlackWell Publishing Ltd 2014
Sujets:
Research Articles
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4122687/
https://ncbi.nlm.nih.gov/pubmed/24571640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1462-2920.12436
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!

Documents similaires