In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry

Here we describe the application of a new click chemistry method for fluorescent tracking of protein synthesis in individual microorganisms within environmental samples. This technique, termed bioorthogonal non-canonical amino acid tagging (BONCAT), is based on the in vivo incorporation of the non-c...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Hatzenpichler, Roland, Scheller, Silvan, Tavormina, Patricia L, Babin, Brett M, Tirrell, David A, Orphan, Victoria J
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: BlackWell Publishing Ltd 2014
Gaiak:
Research Articles
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4122687/
https://ncbi.nlm.nih.gov/pubmed/24571640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1462-2920.12436
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