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DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
The CRISPR-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA:DNA base-pairing to target foreign DNA in bacteria. Cas9:guide RNA complexes are also effective genome engineering agents in animals and plants. Here we use single-molecule and bulk biochemical experiments to determine how...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4106473/ https://ncbi.nlm.nih.gov/pubmed/24476820 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature13011 |
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