Cargando...

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

The simplicity of programming the CRISPR-associated nuclease Cas9 to modify specific genomic loci suggests a new way to interrogate gene function on a genome-wide scale. We show that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique...

Descrición completa

Gardado en:
Detalles Bibliográficos
Main Authors: Shalem, Ophir, Sanjana, Neville E., Hartenian, Ella, Shi, Xi, Scott, David A., Mikkelson, Tarjei, Heckl, Dirk, Ebert, Benjamin L., Root, David E., Doench, John G., Zhang, Feng
Formato: Artigo
Idioma:Inglês
Publicado: 2013
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4089965/
https://ncbi.nlm.nih.gov/pubmed/24336571
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1247005
Tags: Engadir etiqueta
Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!