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High-throughput functional genomics using CRISPR-Cas9
Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenot...
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| Pubblicato in: | Nat Rev Genet |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2015
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4503232/ https://ncbi.nlm.nih.gov/pubmed/25854182 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nrg3899 |
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