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Crystal cryocooling distorts conformational heterogeneity in a model Michaelis complex of DHFR
Most macromolecular X-ray structures are determined from cryocooled crystals, but it is unclear whether cryocooling distorts functionally relevant flexibility. Here we compare independently acquired pairs of high-resolution datasets of a model Michaelis complex of dihydrofolate reductase (DHFR), col...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4082491/ https://ncbi.nlm.nih.gov/pubmed/24882744 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2014.04.016 |
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