Cloning, Overexpression, and Characterization of a Novel Thermostable Penicillin G Acylase from Achromobacter xylosoxidans: Probing the Molecular Basis for Its High Thermostability

The gene encoding a novel penicillin G acylase (PGA), designated pgaW, was cloned from Achromobacter xylosoxidans and overexpressed in Escherichia coli. The pgaW gene contains an open reading frame of 2,586 nucleotides. The deduced protein sequence encoded by pgaW has about 50% amino acid identity t...

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Bibliographische Detailangaben
Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Cai, Gang, Zhu, Songcheng, Yang, Sheng, Zhao, Guoping, Jiang, Weihong
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 2004
Schlagworte:
Enzymology and Protein Engineering
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC404452/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15128530/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.5.2764-2770.2004
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