Cloning, Overexpression, and Characterization of a Novel Thermostable Penicillin G Acylase from Achromobacter xylosoxidans: Probing the Molecular Basis for Its High Thermostability

The gene encoding a novel penicillin G acylase (PGA), designated pgaW, was cloned from Achromobacter xylosoxidans and overexpressed in Escherichia coli. The pgaW gene contains an open reading frame of 2,586 nucleotides. The deduced protein sequence encoded by pgaW has about 50% amino acid identity t...

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Bibliografiset tiedot
Julkaisussa:Appl Environ Microbiol
Päätekijät: Cai, Gang, Zhu, Songcheng, Yang, Sheng, Zhao, Guoping, Jiang, Weihong
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Microbiology (ASM) 2004
Aiheet:
Enzymology and Protein Engineering
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC404452/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15128530/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.5.2764-2770.2004
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