Overexpression of Penicillin V Acylase from Streptomyces lavendulae and Elucidation of Its Catalytic Residues

The pva gene from Streptomyces lavendulae ATCC 13664, encoding a novel penicillin V acylase (SlPVA), has been isolated and characterized. The gene encodes an inactive precursor protein containing a secretion signal peptide that is activated by two internal autoproteolytic cleavages that release a 25...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Appl Environ Microbiol
Autori principali: Torres-Bacete, Jesús, Hormigo, Daniel, Torres-Gúzman, Raquel, Arroyo, Miguel, Castillón, María Pilar, García, José Luis, Acebal, Carmen, de la Mata, Isabel
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2015
Soggetti:
Enzymology and Protein Engineering
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4309716/
https://ncbi.nlm.nih.gov/pubmed/25501472
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02352-14
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