Chemoenzymatic Site-Specific Reversible Immobilization and Labeling of Proteins from Crude Cellular Extract Without Prior Purification Using Oxime and Hydrazine Ligation

In a facile and potentially general method for protein modification at the C-terminus, aldehyde-modified proteins, obtained from enzymatic protein prenylation, react rapidly with hydrazide and aminooxy surfaces and fluorophores at neutral pH and in micromolar concentration ranges of reagents. This s...

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Hauptverfasser: Mahmoodi, Mohammad M., Rashidian, Mohammad, Dozier, Jonathan K., Distefano, Mark D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4037151/
https://ncbi.nlm.nih.gov/pubmed/23839992
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470559277.ch120247
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