Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template

We describe a new rapid and efficient polymerase chain reaction (PCR)-based site-directed mutagenesis method. This procedure is effective with any plasmid and it employs four oligonucleotide primers. One primer contains the desired mutation, the second is oriented in the opposite direction (one of t...

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Detalhes bibliográficos

Publicado no:	J Biomed Biotechnol
Principais autores:	Allemandou, Flore, Nussberger, Jürg, Brunner, Hans R., Brakch, Noureddine
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Wiley 2003
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC400210/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12975535/ https://ncbi.nlm.nih.govhttps://doi.org/10.1155/S1110724303209141
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