Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template

We describe a new rapid and efficient polymerase chain reaction (PCR)-based site-directed mutagenesis method. This procedure is effective with any plasmid and it employs four oligonucleotide primers. One primer contains the desired mutation, the second is oriented in the opposite direction (one of t...

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Detaylı Bibliyografya
Asıl Yazarlar: Allemandou, Flore, Nussberger, Jürg, Brunner, Hans R., Brakch, Noureddine
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Hindawi Publishing Corporation 2003
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC400210/
https://ncbi.nlm.nih.gov/pubmed/12975535
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/S1110724303209141
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