Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template

We describe a new rapid and efficient polymerase chain reaction (PCR)-based site-directed mutagenesis method. This procedure is effective with any plasmid and it employs four oligonucleotide primers. One primer contains the desired mutation, the second is oriented in the opposite direction (one of t...

Fuld beskrivelse

Bibliografiske detaljer

Udgivet i:	J Biomed Biotechnol
Principais autores:	Allemandou, Flore, Nussberger, Jürg, Brunner, Hans R., Brakch, Noureddine
Format:	Artigo
Sprog:	Inglês
Udgivet:	Wiley 2003
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC400210/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12975535/ https://ncbi.nlm.nih.govhttps://doi.org/10.1155/S1110724303209141
Tags:	Tilføj Tag Ingen Tags, Vær først til at tagge denne postø!