載入...
Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template
We describe a new rapid and efficient polymerase chain reaction (PCR)-based site-directed mutagenesis method. This procedure is effective with any plasmid and it employs four oligonucleotide primers. One primer contains the desired mutation, the second is oriented in the opposite direction (one of t...
Na minha lista:
| Main Authors: | , , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Hindawi Publishing Corporation
2003
|
| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC400210/ https://ncbi.nlm.nih.gov/pubmed/12975535 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/S1110724303209141 |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|
載入...