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Rapid and Programmable Protein Mutagenesis Using Plasmid Recombineering

Comprehensive and programmable protein mutagenesis is critical for understanding structure-function relationships and improving protein function. However, current techniques enabling comprehensive protein mutagenesis are based on PCR and require in vitro reactions involving specialized protocols and...

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Bibliografische gegevens
Gepubliceerd in:ACS Synth Biol
Hoofdauteurs: Higgins, Sean A., Ouonkap, Sorel, Savage, David F.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2017
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5650538/
https://ncbi.nlm.nih.gov/pubmed/28707884
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.7b00112
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